If a game did include an option to dislike a female boss because of her gender, the boss probably would treat a female MC different than a male MC because a female worker should understand gender discrimination and that form of self-discrimination would be weird.
But, then again, as @Cataphrak said, that option is really not necessary. The MC shouldn’t be made to automatically love the NPC, but giving a paragraph description of a NPC and then immediately asking if I love/hate them always makes me choose the neutral option because I don’t know them.
But, do players want to experience discrimination when they may deal with it in their real life? Preferability of "non gender segregation" world in CoG
As for writing different gendered NPCs that have to have a static-ish personality, think of people you know or popular people in media. Clementine is a child and a female from the Walking Dead Game who everyone loves even though (or because) she seems so mature and competent, but the other child characters like Duck and Sara are different and unique which makes each person seem different and more real.